Digital Image Analysis: Quantification of in vitro angiogenesis




Angiogenesis is the formation of new capillaries from preexisting blood vessels. The complex process is accomplished by endothelial cells, lining the intimal surface of blood vessels. Cultured endothelial cells were stimulated to form capillary-like structures which were visualized with fluorescence microscopy after staining with calcein-AM and documented as a digital image. The capillary-like structures were quantified after extraction from the background (segmentation) by analyzing the covered area (extract feature), the total capillary length (skeleton), the number of branching points (nodes) and the number of internal holes (meshes).