Quantification of in vitro angiogenesis: skeletonize the extract

 

skeleton

 

The total length of capillary-like structures formed during in vitro angiogenesis represents a further quantitative characterization. Total length is determined after transforming the extract to a skeleton, which is accomplished by a series of morphological operations. The skeleton reflects not only the total length of angiogenic structures, but is also fundamental to determine nodes and meshes.